Coding

Part:BBa_K1989004:Design

Designed by: Shijun Zhao   Group: iGEM16_Peking   (2016-10-12)


Triple-spytag followed by mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 959
    Illegal AgeI site found at 1071
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant.


Source

The length of the linker between spycatcher may affect the physical and chemical property of hydrogel.


References